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Abstract
8 S9 \5 O" F7 [2 f7 M2 R5 ~) t, ^. mAligned microcontact printing for patterning the sample in areas of homogeneous RF-field on the highly sensitive surface of planar% d* b" j+ p% d" o" F3 `
NMR microprobes is presented. We experimentally demonstrate that sample patterning allows drastic improvement of the spin excitation+ W5 P5 v% Q. d! h% l& u
uniformity. The NMR microprobes are designed for cell analysis and characterized using lipid vesicles as cell substitutes. Lipid vesicles. {6 Q9 ?- i$ r! k1 z- J7 i! M
are advantageous as composition and concentration of the confined solution are precisely controlled and because of their similarity3 u* x1 t9 r5 S5 Z' u, A/ a! M& P
to living cells. Using aligned microcontact printing, a monolayer of lipid vesicles is immobilized on the surface of the planar NMR microprobe% H; v9 r* l" }: @) l' R' y
in a patterned way. 1H NMR spectra and CPMG spin echoes of sucrose solution confined within the lipid vesicles are successfully
9 T, W% ?( b- `. `" Urecorded. Nutation curves of the sample structured in different patterns demonstrate the impact of patterning on the spin excitation uniformity." |$ N1 p R0 A
The total detection volumes are between 1 and 2 nL and derived with help of a theoretic model based on 3D finite element simulation.
6 y4 A" c$ ]) \( A# n9 BThis model predicts the signal-to-noise ratio and the progression of the nutation curves.1 m2 V. o" f" g5 b
2005 Elsevier Inc. All rights reserved.
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