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Abstract8 s! o4 Z. ^. N6 e
Aligned microcontact printing for patterning the sample in areas of homogeneous RF-field on the highly sensitive surface of planar9 H, v1 o0 x: D5 [5 H+ G
NMR microprobes is presented. We experimentally demonstrate that sample patterning allows drastic improvement of the spin excitation3 f4 i' M* S; p8 f+ R
uniformity. The NMR microprobes are designed for cell analysis and characterized using lipid vesicles as cell substitutes. Lipid vesicles1 x7 o& L! q% u4 B1 ~* F; U6 d
are advantageous as composition and concentration of the confined solution are precisely controlled and because of their similarity+ T0 Y. {0 q! r' a* z
to living cells. Using aligned microcontact printing, a monolayer of lipid vesicles is immobilized on the surface of the planar NMR microprobe7 M# X M7 I: j- ~2 X2 W# ~
in a patterned way. 1H NMR spectra and CPMG spin echoes of sucrose solution confined within the lipid vesicles are successfully3 ?. C' M& _8 C, J- S4 e( }
recorded. Nutation curves of the sample structured in different patterns demonstrate the impact of patterning on the spin excitation uniformity.% M# b1 L0 d6 M" I8 g
The total detection volumes are between 1 and 2 nL and derived with help of a theoretic model based on 3D finite element simulation.
) \0 C- B! L$ W3 q1 \% ~% I2 I3 CThis model predicts the signal-to-noise ratio and the progression of the nutation curves., o2 B9 S: x9 p
2005 Elsevier Inc. All rights reserved.
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[ 本帖最後由 mt7344 於 2007-6-7 10:38 PM 編輯 ] |
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